Purification

Target
IDP98885  
Purification ID
A23891  
Expression
LB + 40 mM K2HPO4 (Sep 01, 2021)  
Experiment Date Start
Sep 07, 2021  
Experiment Date End
Sep 08, 2021  
Person
Christine Tesar  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
PIG-I, SEC, IMAC-II::concentration and buffer exchange (Amicon 3k or 10k)::PIG IMAC-II (PIG-II) program eluate (40 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
2  
Final Volume (μL)
18000  
Shipped To Lab Id
ANL  
Shipped Date
Sep 09, 2021  
Inserted
Jan 31, 2022