Purification

Target

IDP01178  

Purification ID

A5458  

Expression

M9 minimal (Feb 23, 2011)  

Experiment Date Start

Mar 15, 2011  

Experiment Date End

Mar 16, 2011  

Person

Magdalena Makowska-Grzyska  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC::SEC peak 2::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleavage unsuccessful  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

8  

Final Volume (μL)

1700  

Shipped To Lab Id

ANL  

Shipped Date

Mar 16, 2011  

Inserted

Jul 29, 2011