Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP04619
Purification ID: A2092
Expression: M9 minimal (Feb 02, 2010)
Experiment Date Start: Mar 08, 2010
Experiment Date End: Mar 10, 2010
Person: Magdalena Makowska-Grzyska
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: cleaved
SeMet Status: native
Covalent Modification
Final Buffer: 50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole
Final Concentration (mg/mL): 60
Final Volume (μL): 220
Shipped To Lab Id: ANL
Shipped Date: Mar 10, 2010
Inserted: Jul 29, 2011

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Mar 11, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	Index Screen	65	CrystalQuick standard profile, LBR round bottom plate
Mar 11, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	ANL-2	80	CrystalQuick standard profile, LBR round bottom plate
Mar 11, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	ANL-2	66	CrystalQuick standard profile, LBR round bottom plate
Mar 11, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	ANL-1	18	CrystalQuick standard profile, LBR round bottom plate
Mar 11, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	Index Screen	5	CrystalQuick standard profile, LBR round bottom plate
Mar 11, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	nothing	sitting drop	ANL-1	65	CrystalQuick standard profile, LBR round bottom plate
Mar 11, 2010	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	75	CrystalQuick standard profile, LBR round bottom plate