Purification

Target

IDP91538  

Purification ID

A8062  

Expression

M9 minimal (Dec 13, 2011)  

Experiment Date Start

Jan 12, 2012  

Experiment Date End

Jan 18, 2012  

Person

Min Zhou  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

na  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

191  

Final Volume (μL)

450  

Shipped To Lab Id

ANL  

Shipped Date

Jan 20, 2012  

Inserted

Feb 02, 2012