Purification

Target
IDP00409  
Purification ID
A4922  
Expression
M9 minimal (Oct 15, 2008)  
Experiment Date Start
Nov 20, 2008  
Experiment Date End
Nov 22, 2008  
Person
Rory Mulligan  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
0  
Final Volume (μL)
0  
Shipped To Lab Id
ANL  
Shipped Date
Nov 22, 2008  
Inserted
Feb 27, 2012