Purification

Target
IDP00734  
Purification ID
A3027  
Expression
M9 minimal (Oct 12, 2010)  
Experiment Date Start
Aug 23, 2010  
Experiment Date End
Aug 25, 2010  
Person
Magdalena Makowska-Grzyska  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
2  
Final Volume (μL)
17000  
Shipped To Lab Id
ANL  
Shipped Date
Aug 25, 2010  
Inserted
Feb 27, 2012