Purification

Target
IDP00040  
Purification ID
A3505  
Expression
M9 minimal (Jun 08, 2010)  
Experiment Date Start
Jun 16, 2010  
Experiment Date End
Jun 18, 2010  
Person
Min Zhou  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
3  
Final Volume (μL)
15000  
Shipped To Lab Id
ANL  
Shipped Date
Jun 18, 2010  
Inserted
Feb 27, 2012