Center for Structural Genomics of Infectious Diseases

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Purification

Target: IDP01178
Purification ID: A9223
Expression: M9 minimal (Mar 14, 2012)
Experiment Date Start: Apr 09, 2012
Experiment Date End: Apr 10, 2012
Person: Magdalena Makowska-Grzyska
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: not started
Description: IMAC-I, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: na
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 42
Final Volume (μL): 900
Shipped To Lab Id: ANL
Shipped Date: Apr 10, 2012
Inserted: Apr 19, 2012

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	9	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	17	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	18	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	63	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	40	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	20	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	12	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	12	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	22	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	88	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	89	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	53	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	82	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	84	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	6	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	21	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	43	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	50	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	54	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	61	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	80	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	21	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	79	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	64	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	69	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	21	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	30	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	5	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	20	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	24	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	28	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	34	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	40	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	18	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	22	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	32	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	36	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	62	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	63	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	21	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	21	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	26	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	26	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	50	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	50	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	52	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	60	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	60	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	61	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	64	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	30	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	55	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	5	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	17	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	20	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	24	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	28	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	43	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	17	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	18	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	22	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	32	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	36	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	62	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	96	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	23	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	24	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	28	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	31	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	34	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	64	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	92	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	95	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	96	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-PEG Screen	25	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	9	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	17	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	86	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	34	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	17	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	20	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	40	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	61	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	69	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	82	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	21	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Magdalena Makowska-Grzyska	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-PEG Screen	2	CrystalQuick standard profile, LBR round bottom plate