Expression

Target
IDP00223  
Expression ID
A3654  
Protein Clone
pMCSG7 (Apr 15, 2010)  
Experiment Date Start
Jun 23, 2010  
Experiment Date End
Jun 25, 2010  
Person
Magdalena Makowska-Grzyska  
Lab
Argonne National Laboratory  
Protocol
standard expression_ANL  
Status
success  
Expression Organism
E. coli  
Expression Strain
BL21(DE3)magic  
Media Type
M9 minimal + semet  
Media Volume
2000 mL  
Growth Temperature
37 °C  
Induction Temperature
18 °C  
Induction Reagent
0.5 mM IPTG  
Experiment Type
production  
Expression Level
30.0 %  
Solubility Level
90.0 %  
Inserted
Dec 06, 2011  

Related Purifications

Experiment
Start
Date		 Experiment
End
Date		 Person Lab Protocol Status Affinity
Tag
Status		 SeMet
Status		 Covalent
Modification		 Final
Buffer		 Shipped
To
Lab		 Shipped
Date