Protocol

Abbreviation

UVA_default_crystal_harvesting

Name

UVA_default_crystal_harvesting

Laboratory name

University of Virginia

Type

crystal harvest

Description

Standard Virginia Crystal Harvesting protocol:
You will need: microscope, 2 dewars, small and big with liquid nitrogen, loops in vials, canes, wand, cryos, sample dish
1. Prepare loops - clean them and choose proper sizes
2. Sign vials and canes
3. Bring liquid nitrogen in 2 duars - small with vial's holder and big with long canes
4. Using microscope fish crystal from the well with loop
5. Immerse crystal in chosen cryo
6. Flash freeze crystal in liquid nitrogen
7. Put vial into proper position in cane (position 1 in cane is in the bottom)
8. Store frozen crystals in big dewar, remember to refill it with liquid nitrogen

07/20/2009 Aleksandra Knapik