Purification

Target
IDP00213  
Purification ID
A19044  
Expression
M9 minimal + semet (Oct 29, 2014)  
Experiment Date Start
Nov 18, 2014  
Experiment Date End
Nov 26, 2014  
Person
Gekleng Chhor  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-II program::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
semet  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
1  
Final Volume (μL)
62000  
Shipped To Lab Id
ANL  
Shipped Date
Nov 20, 2014  
Inserted
Jan 29, 2015