Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP91025
Purification ID: A21924
Expression: M9 minimal + semet (Dec 12, 2017)
Experiment Date Start: Dec 12, 2017
Experiment Date End: Dec 14, 2017
Person: Grazyna Joachimiak
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: na
SeMet Status: semet
Covalent Modification
Final Buffer: 50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole
Final Concentration (mg/mL): 135
Final Volume (μL): 750
Shipped To Lab Id: ANL
Shipped Date: Dec 14, 2017
Inserted: Dec 20, 2017

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	9	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	2	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	7	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	14	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	26	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	28	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	31	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	33	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	36	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	38	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	39	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	45	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	51	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	57	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	78	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	86	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	87	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	88	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	93	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	20	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	23	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	45	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	46	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	74	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	75	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	83	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	88	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-2	95	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	8	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	16	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	39	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	64	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	67	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	68	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	69	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	71	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	72	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	86	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	89	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-4	90	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	15	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	16	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	17	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	18	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	20	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	21	CrystalQuick standard profile, LBR round bottom plate
Dec 18, 2017	Grazyna Joachimiak	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	22	CrystalQuick standard profile, LBR round bottom plate